Purification and characterization of protease enzyme isolated from contaminated soil sample

Chithra. S; Hema Shenpagam. N

Volume : III, Issue : XII, December - 2014

Purification and characterization of protease enzyme isolated from contaminated soil sample

Chithra. S, Hema Shenpagam. N

Abstract :

Isolation and partial purification of protease from contaminated soil sample was the main aim of this study. The production medium for organism was optimized by using different pH, Temperature, Carbon and Nitrogen sources. The protease enzyme was highly active and stable from pH 6.0 to 9.0 with an optimum at pH 9. Its optimum temperature was 35�C. The best carbon and nitrogen sources were mannitol and yeast extract. Protease was partially purified by ammonium sulphate precipitation, dialysis. The specific activity of partially purified protease obtained from ammonium sulphate fractionation is found to be 11.42703U/mg and the fractionation is 14.6 fold purified from the crude enzyme preparation yielding 47.89U/mg from the crude protein. This result showed that Bacillus subtilis under study is a good producer of extra cellular protease, which can be beneficial for industries.