Rapid and Reliable Method For Extraction of Total Rna From Blood/ Tissue

ACHALLA NAGA KAMESWARI VALLI SRAVANI; Gurneet Kaur; Shweta Singh; Mritunjay Kumar Singh

Volume : III, Issue : VI, June - 2014

Rapid and Reliable Method For Extraction of Total Rna From Blood/ Tissue

Achalla Naga Kameswari Valli Sravani, Gurneet Kaur, Shweta Singh, Mritunjay Kumar Singh

Abstract :

To study the function of any cell/ tissue, detect certain diseases, for expression studies, it is highly desirable to obtain RNA/mRNA from a homogeneous source. In this method pure RNA was isolated manually, without trizol or use of PBS or any extraction kit. This Denaturing solution is been used, at first it denatures the cell memane and protein. In this method β- mercaptoethanol is added separately so as to reduce disulphide bonds more efficiently and denature the protein. It eliminates the ribonuclease released during cell lysis and prevents the RNA from getting digested during RNA extraction. Total RNA is extracted by this process with a very high intensity and purity. This process is completed within 2 hours. Intense RNA extracted was further used for cDNA synthesis by RT-PCR.