Volume : V, Issue : II, February - 2016

Rapid detection of Multidrug resistance by Line Probe Assay in 100 sputum positive Mycobacterium tuberculosis cases

Dr. Sarjiwan Kaur, Dr. Poonam Sharma, Dr. Sarbjeet Sharma, Dr. Aruna Aggarwal

Abstract :

<p><p><p><p> INTRODUCTION: Multidrug–resistant tuberculosis (MDR–TB) is defined as resistance to two major bactericidal drugs– Isoniazid (INH) and Rifampicin (RIF) with or without resistance to other first line drugs used for the treatment of tuberculosis. It is the result of random genetic mutations in particular genes conferring resistance. METHODS: A total of hundred consecutive sputum smear positive samples of TB suspects attending the Designated microscopic centre ( DMC) of a tertiary care hospital were included in the study. Decontaminated sputa were subjected to Genotype MTBDRplus line probe assay based on multiplex PCR combined with reverse hyidization on nitrocellulose strips, targeting common mutation , yielding results in 2 days. RESULTS: Out of 100 smear positive sputum samples only 4 (4% )showed Multi Drug Resistance(new and retreated cases combined). Among 28 INH resistant isolates, katG mutation occurred in 15(53.57%) of isolates and in InhA gene occurred in 11 of 28 (39.28%) isolates Among 15% rifampicin resistant isolates, the most common mutation was encountered in codon S531L of rpoB gene. CONCLUSION: Accurate diagnosis and identification of resistant strains is essential for early and efficient treatment and control of MDR–TB. Line probe assay is a good tool for rapid detection of known mutations which could be reliable in predicting the response to therapy.</p></p></p></p>

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Dr. Sarjiwan kaur, Dr. Poonam Sharma, Dr. Sarbjeet Sharma, Dr. Aruna Aggarwal Rapid detection of Multidrug resistance by line International Journal of Scientific Research, Vol : 5, Issue : 2 February 2016

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