Volume : VI, Issue : IV, April - 2017

Relevance of HPLC for HbA1c estimation

Anshu Jamaiyar

Abstract :

 Diabetes Mellitus (DM) has become a global epidemic. Diabetes Mellitus (DM) has become a global epidemic whose incidence is increasing and which already affects one in twelve people (1)

HbA1c can be measured by different methods. Enzymatic, boronate affinity chromatographic and cation–exchange high performance liquid chromatographic (HPLC) methods are considered as gold standard methods.Other methods include Immune assay and Capillary electophorosis( 10)

The accuracy of HbA1c methods can be affected adversely by the presence of hemoglobin (Hb) variants or elevated levels of fetal hemoglobin (HbF). The effect of each variant or elevated HbF must be examined with each specific method.

Glycohemoglobin (GHb), measured as HbA1c, is an invaluable tool for monitoring long–term glycemic control, and as such it is a key issue in diabetes care

Glycosylated haemoglobin (HbA1c) has been in use since 1980s as the ‘gold standard‘ for monitoring glycaemic control and as a predictor of diabetic complications. Even though several conditions, such as, haemolytic anaemia (lowers HbA1c) and aplastic anaemia (raises HbA1c) tend to confound and interfere with HbA1c measurement, in most circumstances HbA1c is a valid and reliable index of glycaemic status

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Anshu Jamaiyar, Relevance of HPLC for HbA1c estimation, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-4 | APRIL‾2017

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