ROLE OF IMPRESSION CYTOLOGY IN THE DIAGNOSIS OF OCULAR SURFACE SQUAMOUS NEOPLASIA (OSSN) AT A TERTIARY EYE CARE CENTRE - OUR EXPERIENCE

DR. MOHMED CHAND MOULA; DR. SRILAKSHMI GOLLAPALLI; DR. N.DIVYA; DR. DURGA KHARIDEHAL

Volume : VIII, Issue : II, February - 2019

ROLE OF IMPRESSION CYTOLOGY IN THE DIAGNOSIS OF OCULAR SURFACE SQUAMOUS NEOPLASIA (OSSN) AT A TERTIARY EYE CARE CENTRE - OUR EXPERIENCE

Dr. Mohmed Chand Moula, Dr. Srilakshmi Gollapalli, Dr. N. Divya, Dr. Durga Kharidehal

Abstract :

Ocular Surface Squamous Neoplasia (OSSN) is a spectrum of conjunctival and corneal epithelial neoplasms manifesting as Benign, premalignant and malignant lesions. The Invasive and intraepithelial forms of OSSN are difficult to differentiate clinically. Impression cytology has important role in the routine diagnosis of dysplasia and malignancy in OSSN. It is preferred over biopsy when treatment with non surgical intervention is considered. Impression cytology refers to the application of a cellulose acetate filter to the ocular surface to remove the superficial layers of the ocular surface epithelium. These cells can then be subjected to histological, immunohistological, or molecular analysis. The study was carried out from January 2016–June 2017 at Sarojini Devi Eye Hospital under Osmania Medical College.Hyderabad. 35 cases of biopsy proven intraepithelial and invasive OSSN were included in the study. On cytomorpholigical study fallowing Impression Cytology (IC) 31/35 cases correlated with histolopathology . The histopathological spectrum included 14 cases of invasive SCC, 12 cases of In–situ SCC and 9 cases of Dysplasia. IC using Millipore Cellulose acetate paper showed high correlation 88.5% in detecting OSSN.