RP–HPLC Method Development and Valdation for the Simultaneous Determination of Telmisartan and Atorvastatin in Bulk and Pharmaceutical Dosage form

N. Mukuntha Kumar; Sumathi V rao; Konde Abbulu; B.Venkata Narayana; I. Sukumar

Volume : III, Issue : XI, November - 2014

RP–HPLC Method Development and Valdation for the Simultaneous Determination of Telmisartan and Atorvastatin in Bulk and Pharmaceutical Dosage form

N. Mukuntha Kumar, Sumathi V Rao, Konde Abbulu, B. Venkata Narayana, I. Sukumar

Abstract :

A simple, accurate, precise RP–HPLC method has been developed and validated for the simultaneous estimation of Telmisartan and Atorvastatin in fixed–dosage formulation. The separation was achieved on a C–18 reversed phase column (ACE 5, C–18, 150mm x 4.6mm, 5�) using Acetonitrile: water: Orthophosphoricacid acid (95:5:01 v/v) as mobile phase–A & Acetonitrile: water: Orthophosphoricacid acid (5:95:01 v/v) as mobile phase–B at a flow rate of 1.5mL/min and temperature of 25�C. The UV detection was carried out at 254nm. The retention time of Telmisartan and Atorvastatin was found to be 9.08 and 14.68 min. respectively. The method has been validated for Specificity, Linearity, Accuracy, Precision and Robustness. The caliation curve for Telmisartan and Atorvastatin were linear from the range of 20.01–120.05 �g/mL and 2.5–14.97�g/mL respectively. The mean recoveries obtained for Telmisartan and Atorvastatin were 99.9% and 99.2% respectively. The developed method was found to be Specific, Accurate, Precise, Robust and rapid for the simultaneous estimation of Telmisartan and Atorvastatin in Bulk & Tablets 40mg/10mg & 80mg/10mg.