IJSR - International Journal of Scientific Research

M. Ravishankar; V. Christibai Juliet Ester

Volume : VI, Issue : IX, September - 2017

Screening of Bioactive compounds in Hydrophila auriculata leaves by HPLC, FTIR and UV–Visible Spectroscopy

M. Ravishankar, V. Christibai Juliet Ester

Abstract :

The present study was aims to investigate the Bioactive compounds in Hydrophila auriculata leaves using HPLC, FTIR and UV–Visible Spectroscopy. The results showed the presence of bioactive compounds in aqueous extract of Hydrophila auriculata Vi2 tannin, saponnin, flavonoids,terpenoids, triterpenoids, carbohydrate, polyphenol were present ,while phlobatannins, steroids, alkaloids, protein, anthroquinone and glycoside were absent. Methanol extract of Hydrophila auriculata leaves showed the presence of tannin, saponnin, flavonoids, steroids, alkaloids, terpenoids, triterpenoids, anthroquinone, carbohydrate and polyphenol while phlobatannins, protein and glycoside were absent. Tannin, Saponnin, Flavonoids, Steroids, Alkaloids, Triterpenoids, Polyphenol were present while Phlobatannins, Terpenoids, Carbohydrate, Protein, Anthroquinone and Glycoside were absent in ethanol extract of Hydrophila auriculata leaves. Significant quantity of Phenol (204.23±14.28), Flavonoids (125.78±8.75), Alkaloids (68.77±4.80), Tannin (40.32±2.8) and Terpenoids (58.63±4.06) present in Hydrophila auriculata leaves Results of the UV spectrum indicates the presence of flavonoids , phenol and its derivatives in the Hydrophila auriculata leaves. The results of the FTIR confirmed the presence of alcohol, phenol, Alkenes, Aromatics, Carboxylic acids, Aliphatic amines and Nitro compounds. HPLC profiles of Hydrophila auriculata were analysed and seven phenolic compounds namely Kaempferol, P–coumaric Acid, Epicatechin,Tannic acid, Epigallocatechin, Ellagic acid and Nåenin were identified.. This supports the popular use of Hydrophila auriculata leaves in preparation of various pharmaceutical formulations for human welfare.