Significance of Interleukin6, Interleukin8, Procalcitonin and Creactive protein as early diagnostic markers of sepsis

Mohammad Moniruzzaman; ASM Areef Ahsan; Ashesh Kumar Chowdhury

Volume : VI, Issue : VI, June - 2017

Significance of Interleukin6, Interleukin8, Procalcitonin and Creactive protein as early diagnostic markers of sepsis

Mohammad Moniruzzaman, Asm Areef Ahsan, Ashesh Kumar Chowdhury

Abstract :

Background: Sepsis is a leading cause of death among critically ill patients in Intensive Care Unit (ICU). Rapid diagnosis and timely initiation of effective therapy are the major challenges. Interleukin–6 (IL–6), Interleukin– 8 (IL–8), Procalcitonin (PCT) and C reactive protein (CRP) are the informative laboratory markers in this regard. Objectives: To determine serum levels of IL–6, IL–8, PCT and CRP in patients with sepsis and to see the relation of these markers with various stages of sepsis, also to determine the diagnostic performance of these markers in patients with sepsis. Methods: In this prospective cross sectional study, a total of 80 subjects was enrolled. 60 of them were patients with at least 2 systemic inflammatory response syndrome (SIRS) criteria and 20 age matched people without SIRS criteria. All patients had been selected from the ICU of BIRDEM General Hospital with known clinicopathological parameters. Serum levels of IL–6, IL–8, and PCT were assessed using Emzyme Linked Immunosorbent assay (ELISA) method. Results: Significant difference was found in IL–6, IL–8 and PCT values in the patients with bacteriological culture positive and negative group (p<0.05), but CRP could not draw any differences. There was increasing trend of serum IL–6 and PCT with the developing stages of sepsis. In diagnostic performance test, PCT yields highest diagnostic value than IL–6 and receiver operating characteristics (ROC) curve shows area under the curve (AUC) for PCT was 0.785 (95% CI; 0.654–0.915), sensitivity 89.47%, specificity 50%, positive predictive value (PPV) 75.55% and negative predictive value (NPV) 73.33% with cutoff value >753pg/ml. Conclusion: Our study revealed elevated levels of serum IL–6, IL–8 and PCT in the patients with sepsis. Therefore, combinatorial use of these biomarkers may serve a potential role in early diagnosis of sepsis. However, multi–centric prospective study on large cohort may be undertaken to evaluate the exact influence.