Volume : VI, Issue : VII, July - 2017

SODIUM FLUORIDE VACUTAINER AN ALTERNATIVE TO EDTA FOR ESTIMATION OF GLYCATED HEMOGLOBIN.

Snehankar Kalita, Rumi Deori, Risha Goswami, Kailash Bhattacharyya

Abstract :

 INTRODUCTION: Glycated haemoglobin (HbA1c) has been used primarily as a marker to identify the average amount of plasma glucose concentration over prolonged period of time. As the average amount of plasma glucose increases, the fraction of glycated haemoglobin increases in a predictable way. HbA1c is nowadays used as a prognostic and diagnostic marker for glycemia control in Diabetes Mellitus patients. Most of the commercial kits for HbA1c estimation require whole blood to be collected in EDTA (ethylenediaminetetraacetic acid) anticoagulant, which needs collection of additional blood sample from the patients. If blood sample collected in sodium fluoride vial could be used to estimate blood glucose as well as glycated haemoglobin, collection of additional blood sample from the patient could be avoided.

AIMS AND OBJECTIVES: The present study was designed to determine the effect of common blood additives like sodium fluoride and EDTA on HbA1c level and also to see the variation in values of HbA1c for one week.

MATERIALS AND METHODS: Blood samples were collected in both EDTA and sodium fluoride vials from randomly selected patients of either sex. HbA1c was estimated using HPLC method.

RESULTS AND OBSERVATIONS: For all the samples, the glycated hemoglobin value of the samples collected in EDTA vials showed no significant differences with that of the same samples collected in Flouride vials. It was also observed that the glycated hemoglobin values did not alter significantly within 7 days of collection when stored at 2–8° C in both the vials.

CONCLUSION: The sodium flouride vial used for estimation of plasma glucose can also be used to estimate HbA1c as well without having to collect additional blood sample from the patients, as has to be done when using EDTA vial.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Snehankar Kalita, Rumi Deori, Risha Goswami, Kailash Bhattacharyya, SODIUM FLUORIDE VACUTAINER AN ALTERNATIVE TO EDTA FOR ESTIMATION OF GLYCATED HEMOGLOBIN., INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-7 | JULY-2017

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