Studies on bacteriorhodopsin synthesis in UV induced Mutant Halobacterium salinarium with different time of UV exposure

Nazeerullah.R; Thajuddin.N; Syed Moideen.R

Volume : V, Issue : I, January - 2016

Studies on bacteriorhodopsin synthesis in UV induced Mutant Halobacterium salinarium with different time of UV exposure

Nazeerullah. R, Thajuddin. N, Syed Moideen. R

Abstract :

Experiments on UV irradiation on Halobacterium salinarium was performed under 270 wave length UV source at 6.5 cm distance with different time of exposure from 10 seconds to 8 minutes for bacteriorhodopsin pigment synthesis and phototatic response. BR pigmented phototactic resistant H. salinarium colonies were isolated from 10 seconds to 50 seconds UV exposed culture. White colour, non phototactic thread like mutant colonies were isolated from 1 minute to 6 minutes UV exposed colonies. The results show that the UV radiation directly affects the flagellar movement and BR expression, also quantitative analysis of BR synthesis was done in the cultures. The obtained results which is strongly stated that the UV light affects the BR expression depends on time of exposure and time of intervals.