Studies on Colletotrichum capsici – a Plant  Pathogenic Fungus: Selection of Culture Medium  for Growth And Determination of Minimum  Inhibitory Concentration of Fungicide

Aparajita Roy; Ajay Krishna Saha; Panna Das

Volume : II, Issue : IV, April - 2013

Studies on Colletotrichum capsici – a Plant Pathogenic Fungus: Selection of Culture Medium for Growth And Determination of Minimum Inhibitory Concentration of Fungicide

Aparajita Roy , Ajay Krishna Saha, Panna Das

Abstract :

The present study investigated the growth of Colletotrichum capsici in four different growth medium Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA), Sabouraud Dextrose Agar (SDA) and Peptone Yeast Extract Dextrose Agar (PYDA). Among the different media tested, PDA supported the maximum growth significantly compared to all other media. The two fungicides Indofil M-45 and Carbendazim were evaluated for MIC (minimum inhibitory concentration) activity against the fungi. The effect of Indofil M-45 and Carbendazim on the fungi was evaluated by the disc diffusion method and the inhibition zones showed that the diameter of the colony of the Colletotrichum capsici was reduced. The higher concentration gave the maximum effect which decreased with dilutions. Carbendazim showed maximum efficacy even at a very low concentration of 10-9 mg/ml as compared to that of Indofil at 10-3 mg/ml. The two fungicides Indofil M-45 and Carbendazim were found to inhibit the growth of the fungi. However, Carbendazim was more effective than Indofil