Volume : III, Issue : VII, July - 2014

Studies on L–Asparaginase Producing Bacteria from Soil Samples of Akola District

Vijay Nimkande, Dipali Barate

Abstract :

L- Asparaginase is an extracellular enzyme having potential therapeutic applications. The present work describes the isolation, screening, Identification, enzyme production, protein estimation and characterization of bacteria isolated from various parts of Akola region. Bacteria were screened for L- asparaginase production from farm soil on the basis of formation of pink color zone around the colony. L-asparaginase producing bacteria were further characterized by morphological, physiological and biochemical studies as Bacillus sp, P. aeruginosa, Serratia sp. and S. aureus. Maximum growth was observed at optimum conditions (pH 7, temperature at 37°C and time 72 rrs.) Maximum protein estimated from isolate no. SR 9. The crude enzyme characterized for effect of temperature, pH, metal ion and substrate and enzyme concentration.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Vijay Nimkande, Dipali Barate Studies on L-Asparaginase Producing Bacteria from Soil Samples of Akola District International Journal of Scientific Research, Vol : 3, Issue : 7 July 2014

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