Volume : VI, Issue : V, May - 2017

Studies on Optimization of Amylase Enzyme Isolated from Bacillus spp.

Chandurkar P. A. , Barate D. L.

Abstract :

 The enzyme from microbial sources are more stable and obtained cheaply. Amylases are  among the most important enzyme and are of great significance in present day industry. Bacterial amylase from isolate Ak–3 (Bacillus subtilis) was potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase was done to found optimum kinetic parameters, which increases the enzyme activity. The optimum conditions of an α–amylase activity were experimentally investigated in details. The optimum activity was achieved with 1.0 concentration unit of α–amylase and 1.0% starch concentration in phosphate buffer solution pH 7 at 37°C for 30 mins. The amylase activity was monitored by dinitrosalicylic acid method based on the measurement of maltose. The maximum activity was recorded at pH 7.0, the optimum temperature was found to be 37°C. The metal ion CuSO4 shows maximum activity. The  maximum activity was recorded at 0.9–1 ml substrate concentration. The optimum time course for amylase activity was found to be at 30 mins. and optimum enzyme concentration was recorded at 0.9–1 ml concentration. Among various C and N sources, 0.5% sucrose and 1% Ammonium sulphate showed maximum activity. 0.1% EDTA concentration was found to be optimized for amylase production. The further detail study could benifit for amylase production in industries from the isolate.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Chandurkar P. A., Barate D. L., Studies on Optimization of Amylase Enzyme Isolated from Bacillus spp., INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | Issue‾5 | May‾2017

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