Studies on the optimization conditions of root and callus initiation in Atropa belladonna in in–vitro conditions

Asha Rani N.S; Prasad M.P

Volume : III, Issue : V, May - 2014

Studies on the optimization conditions of root and callus initiation in Atropa belladonna in in–vitro conditions

Asha Rani N. S, Prasad M. P

Abstract :

Atropa belladonna L. is used in traditional treatments for an assortment of conditions including headache, menstrual symptoms, pectic ulcer, histaminic reactions, inflammation and motion sickness. It is used to overcome onchial spasms, whooping cough. It is used for Parkinson�s disease, antidote for snakebite, gastric agent. The plant species is the source of alkaloid atropine (C??H??NO?, d1–hyoscyamine) which has proven to be a cornerstone in the study of autonomic pharmacology. Root and callus cultures have been established from explants of Atropa belladonna. Explants selected for the present study included leaf midrib and petiole region. MS media constituent with hormone NAA was used in the present investigation. Under 25�3�C explants with NAA showed root initiation. Under same conditions explants with KI differentiated into callus and further formation of roots. But roots obtained from explants (with MS+NAA) directly transferred to MS+KI+2% sucrose media showed callus formation under 25�3�C. Initial media without excess sucrose did not give rise to callus or root formation. Use of MS+BA also did not show any initiation of callus at 25�C but other workers point out a lower temperature of 20–24�C for development of callus.