Study of Mycobactin From Different Mycobacterial Species by its Effect on Growth of Mycobacterium Avium Subsp Paratuberculosis

Radhika Syam; Justin Davis Kollannur; Pranabananda Das

Volume : III, Issue : VII, July - 2014

Study of Mycobactin From Different Mycobacterial Species by its Effect on Growth of Mycobacterium Avium Subsp Paratuberculosis

Radhika Syam, Justin Davis Kollannur, Pranabananda Das

Abstract :

Genus Mycobacterium consists of pathogenic and saprophytic organisms who meet the demand of iron by synthesizing mycobactins and exochelins. Mycobacterium avium subsp paratuberculosis (MAP) is unable to synthesize required amount of mycobactin and require exogenous mycobactin. The mycobactins extracted from various mycobacterial species by ethanol and chloroform extraction and purified by aluminium oxide chromatography. M phlei and M tuberculosis gave maximum growth in varying iron concentrations indicating its ability to utilize iron efficiently. Among all the mycobacterial species studied, highest mycobactin production was noticed in M fortuitum. Out of all extracted mycobactins used for the growth assay, mycobactins extracted from M tuberculosis, M phlei and MAP had produced best growth of MAP