The influences of GSTT1 and GSTM1 null genotype as the risk factor in diabetes type 2 complica–tions

Dadbinpour A; Sheikhha MH

Volume : VII, Issue : I, January - 2018

The influences of GSTT1 and GSTM1 null genotype as the risk factor in diabetes type 2 complica–tions

Dadbinpour A, Sheikhha Mh

Abstract :

Diabetes is one of the multifactorial disorders with genetics and environmental factors playing important role in its cause. In diabetes there are defects in cellular metabolism that results in increasing free radicals. These radicals react with other vital cellular molecules which are responsible in diabetes side effects. Human glutathione–S transferases (GST) are a family of enzymes that catalyses conjugation of electrophilic substances with glutathione. This family divided to four classes named alpha (α), mu (μ), teta (θ) and pi (π). Two member of this four classes are GSTT1 and GSTM1.

Material and Methods: In this study we investigated deletion of GSTT1 and GSTM1 genes in 104 diabetics patients with side effect and 58 diabetic peoples without any coplication. DNA was extracted from peripheral blood and then multiplex PCR was performed following agarose gel electrophoresis to detect GSTT1 and GSTM1 null genotypes. Data were analyzed with SPSS software.

Results: The results indicated that there was no significant relationship between GSTT1 null genotypes (c2=0.365, P=0.351, df=1), and GSTM1 null genotypes (c2=1.207, P=0.177, df=1), with side effects of diabetes type 2.

Conclusion: No significant correlation between GSTM1 and GSTT1 null genotype with side effect of diabetes indicate this fact that impair cellular metabolism result in increase free radicals and oxidative pressure, GST null genotypes may result in decrease antioxidant capacity which cause side effects of diabetes but the performance of different classes of GST null genotypes can be tissues specific. Additional studies are required to confirm this study.