The prevalence of Plasmid mediated AmpC ?-lactamase production in Enterobacteiaceae lacking chromosomal AmpC ?-lactamases at J L N Medical college and associated group of hospitals, Ajmer,Rajasthan

Dr Surbhi Mathur

Volume : VII, Issue : VII, July - 2018

The prevalence of Plasmid mediated AmpC ?-lactamase production in Enterobacteiaceae lacking chromosomal AmpC ?-lactamases at J L N Medical college and associated group of hospitals, Ajmer,Rajasthan

Dr Surbhi Mathur

Abstract :

Background:Enterobacteriaceae produces AmpC β–lactamases that make them resistant to commonly used antibiotics. AmpC β–lactamases can be chromosomal–mediated or plasmid–mediated AmpC β–lactamases (PABLs). The present study was undertaken to determine the occurrence of PABLs production in clinical isolates in Klebsiella and proteus species.

Objective: To determine the prevalence of plasmid mediated AmpC β–lactamase production in Enterobacteiaceae lacking chromosomal AmpC β–lactamases.

Methodology: In this prospective study all non–duplicate Enterobacteriacae 100 isolates recovered from various clinical samples submitted in microbiology laboratory of our hospital. Isolated over a period of 3 months were screened for AmpC β–lactamase production.

Results: Among the 100 clinical isolates tested only 21 isolates were found to harbour AmpC enzymes Confirmed by AmpC disk test and cloxacillin inhibitor based method.

Of these isolates , 15 isolates were identify as of Klebsiella spp. and 6 isolates were identify as a Proteus mirabilis.

Conclusion: Some of the AmpC isolates were sensitive to cefoxitin but were either resistant to cefotaxime or ceftazidime or were resistant to both antibiotics.These data indicate that although methods that use cefoxitin in standardised methods to detect AmpC–harbourng isolates,are useful ,they are not perfect. The results in the present study showed that screening should include all the clinical isolates showing resistance to any of the cephalosporins irrespective of their cefoxitin susceptibility status.