To study the effects of various fixatives on Brain Tissues – A histological study

Dr Ved Prakash; Dr. Muktyaz Hussein

Volume : VII, Issue : I, January - 2018

To study the effects of various fixatives on Brain Tissues – A histological study

Dr Ved Prakash, Dr. Muktyaz Hussein

Abstract :

The fixation also in optical differentiation of cells and tissue constituents by altering their refractive indices in varying degrees. Present study aimed to find best fixative for a particular organ, so that the best histological section details can be produced. We studied the effect of five different types of fixatives. An essential part of all histological and cytological techniques is preservation of cells and tissues as they naturally occur. The aim of current study is to see the effect of the following fixatives namely 10% formalin, Buffered 10% formalin, Bouin�s fluid, Zenker�s fluid, Carnoy�s fluid on liver tissues and to observe the optimum result in a particular fixative in H&E sections. There is no single fixative which can be considered as best fixative for all purposes. Best fixative for study of nuclear details is Bouin�s fluid.