Volume : V, Issue : V, May - 2016

Use of ESAT–6 Polymerase Chain Reaction for rapid diagnosis of female genital tuberculosis

Ruchi Singh, Bharti Malhotra, Veena Sharma, Pratibha Sharma, Aradhana Chauhan, Jitendra Tiwari

Abstract :

 Background: Female Genital Tuberculosis (FGTB) has been a diagnostic dilemma for clinicians due to the latency of the organism, asymptomatic and varied presentation in majority of the cases and paucity of an accurate diagnostic modality. Culture methods are still the gold standard in the detection of genital tuberculosis (TB) but slow growth of mycobacteria results in unacceptable delay in diagnosis and treatment. Moreover, acid fast bacilli (AFB) culture on Lowenstein–Jensen (LJ) medium has a low detection rate. Polymerase Chain Reaction (PCR) based molecular methods are rapid and sensitive in diagnosing TB infection. Aims and Objectives: In the present study, we aimed to use ESAT–6 based PCR for detection of female genital TB and to compare the results with conventional methods. Materials and Methods: Endometrium biopsy samples from 50 suspected cases of FGTB from June 2012 to August 2012 were processed by modified petroff ’s method and the deposit was used for detection of mycobacteria by AFB smear, AFB culture on both solid LJ and liquid Middle ook–7H9 (MB7H9) media and ESAT–6 based PCR. Results of PCR were compared with those of conventional methods. Results: Out of 50 samples received, a total of 36% (18/50) were positive by either of the methods used. Overall positivity by AFB smear microscopy was 2% (1/50) and 10% (5/50) by MB7H9 liquid culture, 8% (4/50) by LJ solid culture and 20% (10/50) by ESAT–6 based PCR. Conclusion: PCR is very rapid and sensitive technique for the diagnosis of genital tuberculosis and has utility in providing the results in clinically relevant time and thereby helping to initiate the treatment timely

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Ruchi Singh, Bharti Malhotra, Veena Sharma, Pratibha Sharma, Aradhana Chauhan, Jitendra Tiwari International Journal of Scientific Research, Vol : 5, Issue : 5 MAY 2016


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