Validity of Naked Eye Single Tube Red Cell Osmotic Fragility Test for Detection of B–Thalassemia Trait against High–Performance Liquid Chromatography Method

Dr.Chandrahas Prasad; Dr. Shashi Bhushan Singh

Volume : VI, Issue : XII, December - 2017

Validity of Naked Eye Single Tube Red Cell Osmotic Fragility Test for Detection of B–Thalassemia Trait against High–Performance Liquid Chromatography Method

Dr. Chandrahas Prasad, Dr. Shashi Bhushan Singh

Abstract :

This manuscript is focussed to establish the validity of Naked Eye Single Tube Red Cell Osmotic Fragility test (NESTROFT) against gold standard method i.e., High–Performance Liquid Chromatography (HPLC) method for detection of B–Thalassemia Trait. NESTROFT and HPLC METHOD were applied to blood sample of 84 patients of suspected of cases of B–Thalassemia and other haemoglobinopathies. Out of 84, Beta Thal Trait 13 cases (15.4%) , Delta Beta Thal Trait 9( 10.7%) ,Thal Major 5( 5.9%), HPFH 7( 8.3), Sickle Homo 12 (14.2 %), Sickle Trait 10(11.9%) Sickle Thal Trait 7(8.3%) & IDA 21(25%) cases were detected by HPLC. The NESTROFT test was successful in detecting 12/13 subjects with B–Thalassemia trait. Sensitivity of the test was 92.31 % and specificity was 63.38 %. The test was positive in detecting other haemoglobinopathies like sickle cell disease also. The test proved to be simple, cheap easy to perform and adaptable for mass screening coming close to an ideal screening test for B–Thalassemia trait.