Volume : III, Issue : XII, December - 2013

16S rDNA–RFLP analysis of phylogenetic tree of Rhizobium bacteria

Oraon Vinay, Prasad Bhupendra, Singh Kiran

Abstract :

Soil microorganisms play important role in soil formation, fertility and so also productivity. Nitrogen is an important element to support plant growth. Plants depend on soil microorganisms for fixed nitrogen. Among the nitrogen fixing microorganisms, the role of Rhizobium is quite significant. The aim of this study was to study diversity of Rhizobium spp. in agricultural lands of Madhya Pradesh. Physicochemical properties of soil was studied using standard methods while molecular methods used to study diversity within Rhizobium species. Further, population analysis of Rhizobium species in relation to genetic diversity was carried out using 16S rDNA–RFLP PCR. Rhizobium were identified and genetically by determining the %Guanine plus Cytosine content of the whole genome, followed by Restriction enzyme (Mbo I, Hap II, Taq I) treatment of Polymerase Chain Reaction (PCR) amplified product of 16S rDNA segment was performed. The sequences recognized by the restriction enzymes are distributed at variable intervals in the genome of an organism and also vary in number. The separation carried out by electrophoresis (1.6–2% agarose gel) resulted in specific banding pattern differing within as well as among different species. The technique used was helpful in characterizing Rhizobium isolates to be used as inoculants for improving agricultural land quality of Madhya Pradesh (India).

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Oraon Vinay, Prasad Bhupendra, Singh Kiran 16S rDNA-RFLP analysis of phylogenetic tree of Rhizobium bacteria Indian Journal of Applied Research, Vol.III, Issue.XII Dec 2013

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