Volume : IX, Issue : XII, December - 2019

A comparison of ziehl-neelsen staining and fluorescent microscopy for diagnosis of pulmonary tuberculosis.

Bryan Joel Devaraj

Abstract :

BACKGROUND : Tuberculosis continues to be significant ill–health in our country and is the largest reason for loss in healthy life within the productive age acket. We need rapid methods for bacteriological diagnosis of tuberculosis. Hence the present prospective study was undertaken to see the efficacy of zeihl–neilsen staining in the detection of mycobacterium in sputum sample. METHODS : Early morning sputum samples were collected from patients who were suspected of having tuberculosis at Saveetha Medical College and Hospital,Thandalam.Each sample was processed and subjected to zeihl neilsen and flourescent auramine–o staining. RESULTS: The smear positivity for AFB on the conventional ZN method was 7.47% (12/152) while the positivity increased to 14.69% (23/152) on the modified fluorescent method. Some samples that resulted negative in ZN staining were found to be positive in fluorescent staining. CONCLUSION: Fluorescent staining is found to be more sensitive and easily usable without much strain. The fluorescing bacilli are easily identifiable and less time consuming and hence, it has been advocated to be methods of choice where there are large numbers of sputum smears are to be examined

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Article: Download PDF    DOI : 10.36106/ijar  

Cite This Article:

A COMPARISON OF ZIEHL-NEELSEN STAINING AND FLUORESCENT MICROSCOPY FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS., BRYAN JOEL DEVARAJ INDIAN JOURNAL OF APPLIED RESEARCH : Volume-9 | Issue-12 | December-2019

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