A Detection of Human Bocavirus from Fecal Samples of Indian Children with Acute Gastroenteritis

Neeraj Kumar; S K Mehra; Bharti Malhotra; M. Anjaneya Swamy

Volume : VII, Issue : IV, April - 2017

A Detection of Human Bocavirus from Fecal Samples of Indian Children with Acute Gastroenteritis

Neeraj Kumar, S K Mehra, Bharti Malhotra, M. Anjaneya Swamy

Abstract :

Objectives: Acute gastroenteritis (AGE) is a common illness affecting all age groups worldwide, causing an estimated three million deaths annually. Human bocavirus (HBoV), a newly identified member of the Parvoviridae family is associated with gastrointestinal tract diseases and respiratory infections, mostly of young children. To investigate the epidemiological and genetic variation of HBoV in Jaipur, India, we screened 239 stool samples from children with acute gastroenteritis infection symptoms for HBoV between March 2012 and December 2012.

Methods: In our study, 239 stool samples from children (≤5 years) with acute gastroenteritis were collected. Specimens were screened for HBoV by real–time PCR and other 6 common gastroenteritis viruses by RT–PCR or PCR. We have used specific primer and probes.

Results: HBoV was detected in 5(2.09%) out of 239 samples, mostly from inpatient children. The virus carrier children were 1to 5 years old. The ratio of HBoV positive samples is similar to global results (2.1–5.5%).

Conclusions: The positivity of HBoV is very less (2.09%). HBoV is the third most prevalent virus, after rotavirus and astrovirus, associated with pediatric AGE in this study. It is observed that HBoV is found in mix infection commonly.