A Simple, Rapid, Sensitive and Specific Liquid Chromatography Tandem Mass Spectrometric Method Development and Validation for the Quantification of S–(+) And R–(–) Etodolac in Human Plasma

Dr. Tushar Barot; Hardik Brahmbhatt

Volume : IV, Issue : VIII, August - 2014

A Simple, Rapid, Sensitive and Specific Liquid Chromatography Tandem Mass Spectrometric Method Development and Validation for the Quantification of S–(+) And R–(–) Etodolac in Human Plasma

Dr. Tushar Barot, Hardik Brahmbhatt

Abstract :

A stereo selective liquid chromatography tandem mass spectrometry method was developed using solid phase extraction (SPE) for quantification of R–(–) Etodolac & S–(+) Etodolac in human plasma using SEtodolac D4 as internal standard. The separation was achieved by chiral–AGP (100×4mm) 5μm column and 10mM Ammonium acetate (pH 6.0): Acetonitrile (75:25 V/V) as mobile phase, at flow rate 0.500 mL/min. The ion mode was positive. The detection was carried out by Q3 mass detector. The retention time for R–(–) Etodolac & S–(+) Etodolac were found to be 1.90min and 2.57min respectively and 2.50min for S–Etodolac D4. The total run time of S–(+) Etodolac and R–(–) Etodolac was 4.2 minute. The limit of quantification (LLOQ) of R–(–) Etodolac & S–(+) Etodolac were obtained 0.15 μg/mL and 0.10μg/mL respectively using chiral–AGP (100×4mm) 5μm column and using Q3 Mass detector.