A STUDY ON PRODUCTION OF BIOSURFACTANT BY BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL AND ITS OPTIMIZATION

Dr. KALAIVANI R

Volume : V, Issue : XI, November - 2015

A STUDY ON PRODUCTION OF BIOSURFACTANT BY BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL AND ITS OPTIMIZATION

Dr. Kalaivani R

Abstract :

 Biosurfactants are the structurally diverse group of surface–active molecules synthesized by diverse microbes. Hence, there could probably be a potential chance of producing biosurfactant using locally isolated bacteria originated from petrochemical wastes. Furthermore, their role as anti–adhesive agents against several pathogens indicates their utility as appropriate anti–adhesive coating agents for medical insertional materials prime to reduce a large number of hospital infections without the use of synthetic drugs and chemicals. In the proposed study, Pseudomonas aeruginosa was isolated from petrol contaminated soils at Chennai. The isolated strain was screened for the production of biosurfactant. The various factors affecting production of biosurfactant was assayed, which include temperature, pH, different carbon sources, and different oil substrates. In addition to that, sucrose was found to be better carbon source and olive oil showed better production among the oils used. Finally it was confirmed as glycolipids by FTIR analysis.