Alterations in P53 Gene Expression, Lipid Profile, Total Antioxidant Activity and Semen Picture Induced by Repeated Fried Palm Oil in Rats: Possible Protective Role of Ginger

Abeer G.A. Hassan; Abeer A.I. Hassanin; Saadia A. Ali; El�Anwar A. H; Takao Itakura

Volume : VI, Issue : IX, September - 2016

Alterations in P53 Gene Expression, Lipid Profile, Total Antioxidant Activity and Semen Picture Induced by Repeated Fried Palm Oil in Rats: Possible Protective Role of Ginger

Abeer G. A. Hassan, Abeer A. I. Hassanin, Saadia A. Ali, El Anwar A. H, Takao Itakura

Abstract :

<p>&nbsp;Recurrent using of oils during frying of food is a common trend in houses and commercial sectors. The repeated heated oil which is thermally oxidized may have detrimental effects on biological tissues. This study was conducted to examine the effect of repeated fried palm oil alone or in combination with ginger on serum lipid profile, total antioxidant activity (AOA), semen picture and p53 gene expression in rat tissues. Twenty four male rats were divided into four groups (6 each); control (normal basal diet), FPO (diet+ 15% fresh palm oil), RFO (diet mixed with 15% repeated fried palm oil), RFO+ G (diet + RFO+ ginger 1%). The results showed that the high fat diet (FPO or RFO) cause significant elevation in total cholesterol, phospholipids, HDL&ndash;c and LDL&ndash;c. AOA increased in hepatic tissue but decreased in testicular tissue of rats consumed RFO indicating oxidative stress which seems to affect semen picture dramatically causing a decrease in sperm count, motility% and live&ndash;dead% with an increase in sperm abnormalities %. Moreover, P53 gene expression increased significantly in both liver and testis of rats fed RFO. Ginger succeeded to modulate this effect on p53 expression but failed to modulate the biochemical alterations. It could be concluded that using repeated fried oil have obvious harmful effect for vital tissues like liver and reproductive system but the protective role of ginger need further studies.</p>