Volume : VII, Issue : XII, December - 2017

Analysis of biofilm formation in staphylococci by modified tissue culture plate method.

Shruti Asati, Uma Chaudhary

Abstract :

 Context: Biofilm forming microorganisms are associated with chronic infections and are found to be highly resistant to antimicrobial agents.Staphylococci are one of the important pathogen associated with variety of human infections and are known to produce biofilm. Aim: To detect biofilm formation in clinical isolates of Staphylococcus aureus. To compare antimicrobial resistance in biofilm producing and non–producing isolates. Settings and Design: Prospective observational study conducted in a tertiary care hospital. Materials and methods: Total 166 staphylococci isolates obtained from pus samples were studied for biofilm production by modified tissue culture plate (MTCP) method. Antimicrobial susceptibility testing (AST) was done by Kirby–Bauer disc diffusion method. Results: By modified tissue culture plate method, the biofilm production rate was found to be 66.9%. AST pattern revealed 59.6% isolates were Multi Drug Resistant (MDR).The rate of MDR isolates in biofilm positive isolates was 75.2% while only 39.7% non–biofilm producing isolates were found to be MDR.This difference in resistance pattern was found to be statistically significant. Conclusion: Early identification of biofilm producing strains might help to modify the treatment and prevent relapse of such infection. Appropriate antibiotic selection for the treatment of such biofilm associated infections is extremely important. < clear="all" style="page–eak–before:always; mso–eak–type:section–eak" />

 

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Shruti Asati, Uma Chaudhary, Analysis of biofilm formation in staphylococci by modified tissue culture plate method., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-7 | Issue-12 | December-2017

Number of Downloads : 310

References :