ANTI-CANCER ACTIVITY IN HeLa CELL LINES OF PHYTOSYNTHESIZED GOLD NANOCOMPOUNDS USING ANDROGRAPHIS PANICULATA (NILAVEMBU) - CHARACTERISATION, OPTIMIZATION, PHYTOCHEMICAL ANALYSIS & ANTI MICROBIAL STUDY

Kamala Priya M. R.; Priya R. Iyer

Volume : IX, Issue : II, February - 2019

ANTI-CANCER ACTIVITY IN HeLa CELL LINES OF PHYTOSYNTHESIZED GOLD NANOCOMPOUNDS USING ANDROGRAPHIS PANICULATA (NILAVEMBU) - CHARACTERISATION, OPTIMIZATION, PHYTOCHEMICAL ANALYSIS & ANTI MICROBIAL STUDY

Kamala Priya M. R. , Priya R. Iyer

Abstract :

Andrographis paniculata commonly called as Nilavembu is one of the well known medicinal plant having potential anti viral properties. This plant is used for the synthesis of gold nanoparticles predominantly aiming at spherical morphology and upon synthesis screening the nanoparticles for in vitro anti cancer activity. The synthesis was carried out in room temperature with precursor solution, Chloroauric acid and the green extract. The distinctive color change of the precursor solution, Chloroauric acid, from pale yellow to wine red indicated the initial confirmation of the nanoparticles synthesis. The preliminary confirmation of the nanoparticles was done using UV visible spectrophotometer, which showed the characteristic peak for the synthesized nanoparticles. Further, the imaging of the nanoparticles was carried out under SEM (Scanning Electron Microscope) which revealed the size and shape of the synthesized nanoparticles. The nanoparticles were tested for the Anti–Bacterial efficiency by well diffusion assay which showed defined Zones of inhibition against the tested strains of Bacteria. Further, the anti cancer assay gave an IC50 value for the nanocompound at 150 ng/ml