ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST MULTIDRUG RESISTANT (MDR) ENTERIC HUMAN PATHOGEN

Payal Agrawal; Nikhilesh Kulkarni

Volume : VII, Issue : III, March - 2017

ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST MULTIDRUG RESISTANT (MDR) ENTERIC HUMAN PATHOGEN

Payal Agrawal, Nikhilesh Kulkarni

Abstract :

Silver Nanoparticles (AgNPs) have been used as an antibacterial agent due to its unique physical, chemical and biological properties. The present study was aimed to investigate antibacterial activity of silver nanoparticles in combination with conventional antibiotics against MDR enteric human pathogens viz. E. coli, Klebsiella, Salmonella and Shigella species respectively. The results indicate high prevalence of E.coli species. 10 (55%) in all the clinical and environmental samples tested followed by Klebsiella species 4 (22%), Salmonella species 2 (11%), and Shigella species 2 (11%) respectively. In Antibiotic Sensitivity/Resistant pattern it was observed that out off eighteen enteric bacterial isolates, isolates E7, K3 and Sa1 showed resistant against all the antibiotics tested, with MAR index equal to 1 which indicates the presence of multiple drug resistance (MDR) in them. MIC values of AgNPs against MDR isolate E7and K3 was establish to be 80μg/ml whereas, for isolate Sa1 the MIC value was 70μg/ml. The synergistic effect of antibiotics in conjugation with biologically synthesized AgNPs induce the susceptibility among the tested bacterial cultures; viz. Salmonella followed by Klebsiella and E.coli. The susceptibility was induced in bacterial cultures to near about 20 to 50 percent analyzed antibiotics.