Application of validated Derivative  Spectrophotometric method for estimation of  Duloxetine in tablets

Rajyalakshmi.Ch; Prof. C. Rambabu

Volume : III, Issue : VII, July - 2013

Application of validated Derivative Spectrophotometric method for estimation of Duloxetine in tablets

Rajyalakshmi. Ch, Prof. C. Rambabu

Abstract :

Two simple, sensitive, precise and economical UV– spectrophotometric methods have been developed for the determination of Duloxetine in tablet formulation. Method A is simple and direct UV spectrophotometric method and is based on determination of Duloxetine in methanol at 290nm. Method B is first order derivative spectrophotometric method and involved estimation of Duloxetine in methanol using the first–order derivative technique at 274 nm as maxima and 300 nm as minima. Linearity was obtained in the concentration range of 9– 15 μg/ml. These methods were validated as per ICH guidelines and are successfully applied to pharmaceutical formulations because no interferences from tablet excipients were found. The proposed methods were found to be simple, sensitive, accurate, precise, rapid and economical for the routine quality control application of Duloxetine in pharmaceutical formulations.