IJAR - Indian Journal of Applied Research

Manpreet Kour; I Soni; Rajni Sharma

Volume : IV, Issue : I, January - 2014

Assessment of biofilm formation by Enterococcus faecalis causing nosocomial infections and their statistical analysis

Manpreet Kour, I Soni , Rajni Sharma

Abstract :

The aim of the study is to evaluate three method for detection of biofilm formation in Enterococcus faecalis. For detection of biofilm formation 198 clinical isolates of Enterococcus faecalis were observed by Tissue culture plate (TCP), Tube method (TM) and Congo Red agar (CRA) method. Out of the 198 Enterococcus faecalis 149(75%) displayed a biofilm positive phenotype under the optimized condition.TCP method were further classified as high 160(80.8%), moderate 28(14.1%) and weak 10(5.05%) isolates or non biofilm producer. During observation it was seen that TM and CRA did not correlated with TCP method for detection of biofilm formation in Enterococcus faecalis. The TCP method was found to be most 94% sensitive, 83% accurate and reproducible screening method for detection of biofilm production by Enterococcus faecalis.