Assessment of inflammatory markers; IL–8 and TNF–Î± as predictors of atherosclerotic progression of coronary arteries in Egyptian patients with or without diabetes

Nearmeen M. Rashad; Alaa A. Farag; Hassan M. Hassanin; Amal S. Elâ€“Shal; Amer A. Ateya

Volume : VI, Issue : V, May - 2016

Assessment of inflammatory markers; IL–8 and TNF–Î± as predictors of atherosclerotic progression of coronary arteries in Egyptian patients with or without diabetes

Nearmeen M. Rashad, Alaa A. Farag, Hassan M. Hassanin, Amal S. El Shal, Amer A. Ateya

Abstract :

Background: Cardiovascular complications are the leading cause of morbidity among patients with diabetes, and ischemic heart disease is the most common cause of death. Several inflammatory mediators have been proposed to contribute to the pathogenesis of atherosclerosis. The aim of this study was to evaluate the levels of interleukin –8 (IL–8) and tumor necrosis factor–alpha (TNF–α) in Egyptian patients with coronary artery disease. Moreover, we aimed to clarify the possible relationships of IL–8, TNF α , diabetes, as well as other cardiovascular risk factors in Egyptian cardiac patients with different degrees of coronary artery occlusion. Methods: One hundred healthy control subjects, age ,sex and smoking habits were matched with patients (n=100). A retrospective analysis was conducted on patients with coronary artery disease ( CAD), who underwent coronary angiography at cardiology department of Zagazig University Hospitals. The enrolled patients were divided into two groups : diabetic cardiac patients (n=45) and non–diabetic cardiac patients (n=55). Both diabetic and non–diabetic cardiac patients were further subdivided into four groups according to number of vessel occlusion. IL–8 and TNF–α were measured using immune–enzymatic ELISA kits. RESULTS: Diabetic patients with CAD had significantly high values of clinical and biochemical parameters of metabolic syndrome. Also, IL–8 and TNF α levels were significantly higher in diabetic cardiac patients compared to nondiabetic ones. Moreover, when we stratified cardiac patients according to coronary artery occlusion we found that patients with three vessel occlusion had higher levels of inflammatory markers; IL–8 and TNF α, and for further evaluation, IL–8 and TNF α levels in cardiac patients were independently correlated with LDL, fasting blood glucose, waist / hip ratio ,fasting insulin, HbA1c and HOMA–IR by linear regression analysis. Conclusion: IL–8 and TNF–α levels of cardiac patients were associated with diabetes, and may be used as surrogate markers of CAD in diabetes and as strong predictive factors for progression of atherosclerosis in the coronary arteries.