Association between Vitamin (D) Receptor Gene Polymorphisms and Cardiovascular disease in Saudi Population

Abeer S.Al–ghamdi; Archana P.lyer; Munazza gull; Mohammed Nabil Alama

Volume : VII, Issue : V, May - 2017

Association between Vitamin (D) Receptor Gene Polymorphisms and Cardiovascular disease in Saudi Population

Abeer S. Al Ghamdi, Archana P. Lyer, Munazza Gull, Mohammed Nabil Alama

Abstract :

Objective : To investigate three polymorphic sites ApaI,BsmI and TaqI of vitamin D receptor (VDR) gene on chromosome 12q (12–12q14) as candidate Cardiovasculrr disease (CVD) in Saudi Population. Method :A sample of 100 Saudi classified as healthy (control,N= 50 (without CVD) or with CVD (N= 50) was investigated the relationship of VDR gene polymorphisms at three restriction sites ApaI, BsmI and TaqI to the risk of CVD .Blood samples were taken and DNA was extracted from whole blood. The target part of VDR gene was isolated and amplified by the polymerase chain reaction (PCR). PCR products were digested by restriction enzymes: ApaI, TaqI, and BsmI and electrophoresed on agarose gel. Result: The difference in Vitamin D Total (Vit.D) values between each two shows non–significante difference p–value =0.1627. However,we observed that was distribution of genotypes frequency of the ApaI VDR gene polymorphisms differed highiy significantly in CVD patients (p–value =0.0085), but was no significant difference in control or between CVD patients and control groups. On the other hand, there was no significant difference in genotypes and alleles frequencies of the VDR gene polymorphisms at position BsmI and TaqI between CVD patients and control groups in Saudi population. Conclusion:The VDR gene polymorphisms at one restriction sites ApaI to the risk of CVD in Saudi population.