Association between vitamin D receptor gene polymorphisms and Hypothyroidism in Saudi  population.

Eman J.Al–Zahrani; Archana P.lyer; Munazza Gull

Volume : VII, Issue : V, May - 2017

Association between vitamin D receptor gene polymorphisms and Hypothyroidism in Saudi population.

Eman J. Al Zahrani, Archana P. Lyer, Munazza Gull

Abstract :

Objective : To investigate three polymorphic sites ApaI,BsmI and TaqI of vitamin D receptor (VDR) gene on chromosome 12q (12–12q14) as candidate for hypothyroidism (HT) in Saudi population. Method :A sample of 100 Saudi classified as healthy (control,N= 50 (without HT) or with HT (N= 50) was investigated the relationship of VDR gene polymorphisms at three restriction sites ApaI, BsmI and TaqI to the risk of HT .Blood samples were taken and DNA was extracted from whole blood. The target part of VDR gene was isolated and amplified by the polymerase chain reaction (PCR). PCR products were digested by restriction enzymes: ApaI, TaqI, and BsmI and electrophoresed on agarose gel. Result: Thyroid–stimulating hormone (TSH) levels were statistically higher in HT than controls (P–value= 2.44E–10*). The difference in Vitamin D Total (Vit.D) values between each two shows significant difference p–value =0.0207<0.05. However,we observed that were distribution of genotypes frequency of the BsmI TaqI and ApaI VDR gene polymorphisms differed significant between HT and control groups (p= .0127<0.05) for BsmI (p=.0150<0.05) for TaqI and (p=.0376<0.05) for ApaI. Conclusion:The VDR gene polymorphisms at all three restriction sites BsmI,TaqI and ApaI were associated with the risk of hypothyroidism in Saudi population.