BACTERIOLOGICAL PROFILE OF PLEURAL FLUID IN EMPYEMA THORACIS

DR SONIYA SAXENA; DR DHARMENDRA KUMAR MEKLE; DR HEMANT KUMAR MAHOR; DR LOKENDRA DAVE; DR NISHANT SRIVASTAVA

Volume : VI, Issue : VII, July - 2016

BACTERIOLOGICAL PROFILE OF PLEURAL FLUID IN EMPYEMA THORACIS

Dr Soniya Saxena, Dr Dharmendra Kumar Mekle, Dr Hemant Kumar Mahor, Dr Lokendra Dave, Dr Nishant Srivastava

Abstract :

INTRODUCTION– In para pneumonic effusion, empyema is the common problem during natural course. Studies of pleural fluid samples using standard culture and molecular techniques have demonstrated that it is associated with increased prevalence of bacteria. METHODOLOGY–100 clinically diagnosed cases of empyema of age ≥15 years were recruited. Pleural fluid samples was processed by conventional methods.Preparation of media, reagents, Gram staining, identification of culture isolates and antibiotic sensitivity tests were carried out following standard laboratory methods. RESULT– The prevalence of empyema was most common in age group of 26 to 45year (48%) with ratio between male and female of 2.57:1. Streptococcus pneumoniae was the commonest bacteria i.e. in 19%cases, followed by Staphylococcus aureus isolated in 16 % cases, klebsiella in 9% cases, bacteroids in 9%cases, S.milleri in 8% cases, Pseudomonas aeruginosa in 8% cases, E.coli in 8% cases, hemophillus influenzae in 4% cases, peptostreptococcus in 4 % cases, S.pyogenous in 3% cases, and fusobacterium in 2% cases. Gram positives were most sensitive to cefoxitin (45%) followed by ceftarolin (42%) and vancomycin (41%).Gram negative isolates were most sensitive to meropenem(29%) followed by imepenem (29%) and cefepime (24%). CONCLUSION–pleural fluid examination is a useful diagnostic tool to study the aetiology of bacterial empyema. Antibiogram helps in screening resistant pathogens and selecting better drug for treatment, thereby helping to decrease the mortality and morbidity