Biochemical Studies on Serum Adeponectin Level as Predictor to Interferon Alpha Therapy in Egyptian Chronic Hepatitis C Patients.

Mohamed S. Elghareeb; Gamil M. Abd-Allah; Lamis G.Ashour

Volume : V, Issue : II, February - 2015

Biochemical Studies on Serum Adeponectin Level as Predictor to Interferon Alpha Therapy in Egyptian Chronic Hepatitis C Patients.

Mohamed S. Elghareeb, Gamil M. Abd 45 Allah, Lamis G. Ashour

Abstract :

Aim of study: To investigate serum level of Adeponectin compared with response of interferon (IFN)–α treatment; in three stages among Egyptian patients with chronic hepatitis C virus infection. Subjects and Methods: This study was performed on 30 subjects admitted to endemic diseases unit of the Cairo Fatimic Hospital (CFH), from which 20 patients suffering from chronic hepatitis C and 10 healthy volunteers as normal control group. Levels of Adeponectin, AST, ALT, Total Bilirubin, Creatinine, HCV RNA and CBC were determined in sera and blood samples of individuals at the time of admission (before beginning of treatment), four weeks and twelve weeks later (after beginning of treatment). Adeponectin was determined using enzyme–linked immunosorbent assay (ELISA) method. AST, ALT, T.Bilirubin and Creatinine were determined spectophotometrically and HCV RNA was determined by polymerase chain reaction assay (PCR) method. Results: Adeponectins serum level at three stages of treatment were high by significant in week zero (P=0.0053) and decreased significantly in both week four (P=0.0001) and week twelve (P=0.0375), while serum level of the following parameters AST, ALT, Total Bilirubin and Creatinine showed a gradual decrease with treatment time (P >0.1). Regarding three groups of treatment, serum HCV RNA show significance of difference between three samples (P 0.08 in W0, 0.2 in W4 and > 0.1 in W12). Conclusions: This study concluded that, HCV may directly affect adiponectin. This is further supported by the significant increase in adiponectin at the end of treatment. Serum adiponectin at baseline appears to be an independent predictor of liver steatosis and for the achievement of the end of treatment virological response