Biodegradation of bromophenol blue and bromocresol green by laccase enzyme from Malus domestica.

Revathi.S; Mohsin Taj; Shalini.N.S; Sarina.P.Khabade

Volume : X, Issue : VIII, August - 2020

Biodegradation of bromophenol blue and bromocresol green by laccase enzyme from Malus domestica.

Revathi. S, Mohsin Taj, Shalini. N. S, Sarina. P. Khabade

Abstract :

The rapid increase in industrialization and urbanization across the globe has increased the harmful contaminants into our ecosystem. One such is the synthetic dyes from textile industries. The synthetic dyes from these industries contaminate the water bodies causing water pollution and is a major environmental hazard. The Laccase (E.C.1.10.3.2) is a potent enzyme that catalyzes the degradation of synthetic dyes. It is extensively studied in microorganisms. Not much is explored from plant sources. In our current study, we have isolated laccase from Malus domestica (fruit pulp of apple). The substrate used for laccase was guaiacol. The maximum enzyme activity was found to be 2.67U/ml. The kinetic parameters were studied and optimum conditions were pH – 7, temp was 26°C, time– 30 min. The crude enzyme was subjected to a series of purification steps that included ammonium sulphate precipitation followed by dialysis and the dialyzed sample was subjected to anion exchange chromatography using DEAE cellulose. The decolorization of dye was seen after incubation of 72hrs. Complete degradation was accomplished in 8 days.