Volume : III, Issue : VI, June - 2013

Biodegradation of P–Nitro Phenol by an Actinomycete

Reshma Jaweria, S. A. Peshwe, A. G. Ingale

Abstract :

p–Nitrophenol (PNP), a major nitroaromatic xenobiotic is released into the environment as a result of its widespread use and as a eakdown product of organophosphate (OP) agricultural pesticides such as parathion and methyl parathion. It is highly toxic to soil microflora and other non–target organisms. Two strains of actinomycetes A1 and A5 isolated from premises of pesticide industries were found to be promising PNP biodegraders, These srains could degrade up to 300 and 400 mg/L PNP respectively,remarkably high concentrations as PNP is quite toxic even at low concentrations. As strain A5 seem promising, it was subjected to phenotypic and genotypic characterization. The phenotypic characteristics of this strain was typical of actinomycetes. 16S recombinant deoxyribonucleic acid (rDNA) sequence analyses indicated strain A5 to have the highest level of sequence similarity with Streptomyces coeruleorubidus strain HBUM174910, EU841625.1(99%).Further studies on optimization, kinetics and pathways of biodegradation can improve the biodegrading capacities of these actinomycete strains.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Reshma Jaweria, S. A. Peshwe, A. G. Ingale Biodegradation of P-Nitro Phenol by an Actinomycete Indian Journal of Applied Research, Vol.III, Issue.VI June 2013

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