Biosurfactant Production by New Microbial  Isolates from Soil Co– Contaminated With Lube Oil  and Distillery Spent Wash

Kirti V. Dubey

Volume : III, Issue : IX, September - 2013

Biosurfactant Production by New Microbial Isolates from Soil Co– Contaminated With Lube Oil and Distillery Spent Wash

Kirti V. Dubey

Abstract :

Present work presents utilization of industrial wastes such as distillery spent wash, curd whey, fruit processing waste and sugar industry effluent for cost–effective production of biosurfactant by four new microbial isolates designated as BS–A, BS–J, BS–K and BS–P isolated from soil sample contaminated with lube oil and distillery spent wash) collected from a distillery unit. These isolates have the potential to produce biosurfactant from mineral salt medium and also from individual wastes viz. distillery waste, sugar industry effluent, fruit processing waste and curd whey waste. Results have shown that highest biomass and biosurfactant yields were obtained in curd whey followed by distillery waste, fruit processing waste and sugar industry effluent by all the four isolates. The surface tension of the fermented wastes reduced from an initial range of 56–60 mN/m to 27–39 mN/m. The fermented wastes showed good emulsification property and the emulsification index (E24) obtained was in the range of 51–54%. Biosurfactant yields obtained from individual wastes were in the range of 0.0043–1.1631 g/l. Reductions in pollutional load of the wastes were observed as total nitrogen, phosphate, and chemical oxygen demand (COD) levels reduced significantly during biosurfactant production by these isolates. This study has shown that these newly isolated biosurfactant producers can find their application in costeffective production of biosurfactant from different types of industrial wastes without supplementing with costly nutrients.