IJAR - Indian Journal of Applied Research

Nehad S Alsubhi; Sarah A Alobaid

Volume : VI, Issue : VI, June - 2016

Cellular and Molecular Biology Effect of Carboplatin and Doxorubicin on Human Endometrial Epithelial HEC�1�B Cell Cultures

Nehad S Alsubhi, Sarah A Alobaid

Abstract :

 Chemotherapy is the main approach in dealing with advanced and recurrent endometrial cancer. This study evaluated the antitumor effects such as carboplatin and doxorubicin on the human endometrial epithelial HEC–1–B cell line. HEC–1–B cells were allowed to grow in DMEM/F12 media with 10% fetal bovine serum and treated with the compounds mentioned above. The target of this research was to identify the exact concentration of two components that HEC–1–B cells were affected in. In the apoptosis assay, it was found that the minimum concentration to induce apoptosis was 2,220 nM. Moreover, a soft agar assay was used to perform which concentration of carboplatin and doxorubicin had the more colonies of cells.