Volume : V, Issue : VII, July - 2015

CFW (Calcofluor White Stain): A Rapid Detection System for Nosocomial Fungal infection

Dr. Shoeb Akhtar Khan, Dr. Immaculata Xess, Dr Amardeep Rai

Abstract :

 Fungal infections in critically ill patients are an increasing prevalent problem. Candida spp cause the majority of these infections in ICU. They occur most commonly in–patients with severe underlying illness, multiple courses of antibiotics and patients with intravascular catheters. Clinical diagnosis of fungal infections in these patients are difficult due to non–specific symptoms. Therefore we compare the detection of fungal elements in nonochoscopic ochoalveolar lavage sample using Calcofluor white stain (CFW) and conventional KOH stain method with culture. Total 432 no BAL samples were collected from total of 80 patients on ventilator for more than 48 hours developing signs and symptoms of nosocomial pneumonia. The sensitivity and specificity of KOH conventional staining technique were 41% and 100% respectively, where as Calcofluor white stain showed sensitivity of 75.2% and specificity of 100% for direct demonstration of fungal elements. All direct positive samples by both techniques were positive by culture 85 (19.6%). The Sensitivity and Specificity of CFW staining for direct demonstration of fungi in clinical specimen superior than the conventional technique.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dr. Shoeb Akhtar Khan, Dr. Immaculata Xess, Dr Amardeep Rai CFW (Calcofluor White Stain): A Rapid Detection System for Nosocomial Fungal infection Indian Journal of Applied Research, Vol.5, Issue : 7 July 2015

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