Characterization of Endophytic Bacteria Isolated from the Medicinal Plant Capparis sinaicaVeill. and Analyze its Bioactive Flavonoid

Magdy Mohsen Mohammed Bahgat; Mona Mahmoud El Bous; Salwa Ali Kawashty; Nesma Ahmed Mohammed El–TabeeYoughly

Volume : IV, Issue : XI, November - 2014

Characterization of Endophytic Bacteria Isolated from the Medicinal Plant Capparis sinaicaVeill. and Analyze its Bioactive Flavonoid

Magdy Mohsen Mohammed Bahgat, Mona Mahmoud El Bous, Salwa Ali Kawashty, Nesma Ahmed Mohammed El Tabeeyoughly

Abstract :

The present work focused on isolation, visualizing endophytic bacteria in its natural niche, inside plant tissue,and identification of cultivableendophytic bacteria that colonize aerial parts ofCapparissinaica, as anindigenous plant to Sinai&amp;ndash;Egypt. C.sinaicais a medicinal plant was used in the treatment of several human diseasesin traditional medicines.The morphological, biochemical and 16Sr RNA gene sequence analysisrevealed thatBacillus species were the most dominantendophytic bacteria.Mixed microbial culture system of isolated endophyticbacterial species wasused for production of secondary metabolites.Paper Chromatography and HPLC analysis showed that bacterial extract producedquercetin andquercetin 3&amp;ndash;O&amp;ndash;glucopyranoside which were detected in plant extract. Hence, it was concluded that endophytic bacteria produced flavonoid compoundsin vitro similar to that originally produced by the plant itselfconfirming theidea of considering endophytes as the potential sources of novel natural products.