Volume : V, Issue : VII, July - 2015

Characterization of Mycobacteriain HIV/AIDS Patients

Kanwalpreet Kaur, Pushpa Devi, Kanwardeep Singh

Abstract :

 Background: Mycobacterium tuberculosis (MTB) is the most common life threatening opportunistic pathogen infecting HIV/AIDS Patients in developing countries. Recent studies have shown that incidence of non tuberculous mycobacteria (NTM) is also on the rise. Tuberculosis (TB) and HIV co–infection has put a huge pressure on health care facilities as there are many challenges in its diagnosis and treatment. Differentiating NTM from MTB is of utmost value as treatment strategies are totally different in these cases. Methods:In this prospective study of one hundred and six HIV seropositive individuals presenting with symptoms of pulmonary or extra–pulmonary TB, appropriate samples were collected and processed. The organisms were identified by microscopy(Ziehl–Neelsen staining)for acid fast bacilli (AFB), conventional culture [(Lowenstein–Jensen (LJ. media)] and automated culture(BACTEC MGIT 320) methods. MTB and NTM were differentiated by conventional methods and immunochromatographyassays using MPT64 antigen. Results: Twenty two Mycobacteria were isolated from one hundred and six samples. Ten cases were positive on ZN staining, twelve on LJ where as nineteen were positive on MGIT. Out of 22 isolates 19 were M. tuberculosis and 3 were NTM (M.aviumcomplex 2 andM. fortuitum 1). Conclusions: MGIT is better thanLJ medium & ZN stainingto detect Mycobacteria.MPT64 appears reliable with excellent sensitivity and specificityto differentiate between MTB and NTM.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Kanwalpreet Kaur, Pushpa Devi, Kanwardeep Singh Characterization of Mycobacteriain Hiv/Aids Patients Indian Journal of Applied Research, Vol.5, Issue : 7 July 2015

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