Volume : IV, Issue : III, March - 2014

Chemical Analysis of Urinary Cotinine in Subjects Exposed to A Mixture of Contaminants

Iulia Neamtiu, Irina Dumitrascu, Carmen Roba

Abstract :

Several analytical methods have been described in the literature for the analysis of cotinine in urine samples, such as gas chromatography using flame ionization detection, mass spectrometry or nitrogen–phosphorous detector, high performance liquid chromatography with ultraviolet or mass spectrometry detector, capillary electrophoresis, radioimmunoassay and enzyme linked immunosorbent assay. Chromatographic techniques are preferred over the other types of analytical methods because of their high sensitivity and specificity. In our study, we measured cotinine as a biomarker of exposure to environmental tobacco smoke. Spot urine samples were collected and kept frozen at –20°C until performing the analysis. Cotinine was measured in urine by gas chromatography – mass spectrometry (GC–MS) analytical method. The sensitivity of the GC–MS procedure was estimated as the limit of detection calculated for cotinine, at 1.37 µg/L. The accuracy of the GC–MS method was 3.02%. 

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Iulia Neamtiu, Irina Dumitrascu, Carmen Roba Chemical Analysis of Urinary Cotinine in Subjects Exposed to A Mixture of Contaminants Indian Journal of Applied Research, Vol.IV, Issue. III

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