Volume : V, Issue : VIII, August - 2015

CHROMATOCLASTIC EFFECTS OF AQUEOUS EXTRACT OF ORTHOSIPHON THYMIFLORUS AND O. ARISTATUS ON ALLIUM CEPA ROOT TIP CELLS.

Seema Devi Retnamma, John Ernest Thoppil

Abstract :

Cytotoxicity of the aqueous extract of two valuable medicinal plants, Orthosiphon thymiflorus and O. aristatuswas investigated using Allium assay.The roots of A. cepa were exposed to different concentrations of the aqueous leaf extracts (0.02%, 0.05 %, 0.075% and 0.10 %), for four different time durations(½ h, 1 h, 2h, and 3h), using distilled water and Methyl Parathion(MP)as the negative and positive controls. The slides were scored for mitotic index (MI), percentage of chromosome aberration(CA). Statistical analysis was done by student’s t test and one way ANOVA. Significant inhibition of MI, inductions of AC,MN, binucleated cells (BN) and diversified clastogenic and non–clastogenic aberrations were observed in both plant extracts. At higher concentrations and longer duration,O. aristatus showed more cytotoxic potential. Further phytochemical analysis is recommended before using these plants in medicinal preparations and other commercial products.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Seema Devi Retnamma, John Ernest Thoppil Chromatoclastic Effects of Aqueous Extract of Orthosiphon Thymiflorus and O. Aristatus on Allium Cepa Root Tip Cells. Indian Journal of Applied Research, Vol.5, Issue : 8 August 2015

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