Volume : V, Issue : II, February - 2015

Cloning, sequencing and BLAST analysis of kDNA minicircles from Leishmania donovani AG83.

Asit Kumar Chakraborty, Subhankar Bhattacharya, Sourav Kumar Nandi

Abstract :

kDNA in kinetoplastid microbes are unique due to presence of few 30kb maxicircle mitochondrial DNA inter–catenated with 10,000 of ~0.8–1.4kb minicircle plasmids. kDNA minicircles from Leishmania donovani AG83 was digested with PstI restriction enzyme and was cloned into pBluescriptSK+ plasmid vector. The cloned minicircle DNA was sequenced by di–deoxy method giving 837bp circular sequence. BLAST analysis suggested the infectious clone minicircle was very similar conserved UMS sequence involved in DNA replication but it had very canonical TATA–box and gRNA sequences involved in ND2/ND3 gene editing. Major sequence variations with respect to UR6 were found between nucleotides 31–41 and 434–444. We are first to show kDNA minicircle sequence of any infectious clone of Leishmania of Indian origin.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Asit Kumar Chakraborty, Subhankar Bhattacharya, Sourav K. Nandi Cloning, sequencing and BLAST analysis of kDNA minicircles from Leishmania donovani AG83. Indian Journal of Applied Research, Vol.5, Issue : 2 February 2015

Number of Downloads : 699

References :