Coelomic Fluid of Earthworm (Eudrilus eugeniae) as Antibacterial Agent Against Vibrio alginolyticus and Staphylococcus hominis

Padmashree Kulkarni; Prof. GeetaKaicker; Dr. Radha D. Kale

Volume : IV, Issue : XII, December - 2014

Coelomic Fluid of Earthworm (Eudrilus eugeniae) as Antibacterial Agent Against Vibrio alginolyticus and Staphylococcus hominis

Padmashree Kulkarni, Prof. Geetakaicker, Dr. Radha D. Kale

Abstract :

<p>Coelomic fluid (CF) of earthworm Eudriluseugeniae was collected and its antibacterial activity was tested< /> against two strains of bacteria Viio alginolyticus and Staphylococcus hominis procured from IMTECH,< /> Chandigarh. The gut of the earthworm was cleared by feeding on filter paper and CF was collected by chemical method. Dilution assay, agar dilution assay, disc diffusion assay and agar diffusion assays were carried out to test the antibacterial activity. Results indicate the suppression of growth of both the strains of bacteria. However, response of S.< /> hominiswas better than that of Viio. Dilution assay proved to be the most sensitive method for antibacterial comes in< /> direct contact with CF during the assay.</p>