Comparative Impact of Physico–Chemical and Nutritional Parameters on some Phytopathogenic Fungi Isolated from the Phyllosphere of Diseased Tea Leaf (Camellia sinensis L. O. Kuntze).

JAHANARA BEGAM; G. D. SHARMA

Volume : V, Issue : I, January - 2015

Comparative Impact of Physico–Chemical and Nutritional Parameters on some Phytopathogenic Fungi Isolated from the Phyllosphere of Diseased Tea Leaf (Camellia sinensis L. O. Kuntze).

Jahanara Begam, G. D. Sharma

Abstract :

The present study has been evaluated to observe the comparative impact and susceptibility in different physicochemical and nutritional parameters on the mycelial growth of some phytopathogenic fungi viz.; Pestalotiopsis sp, Colletotrichum gloeosporioides, F. solani, Alternaria alternata and Cladosporium cladosporoids isolated from the phyllosphere of diseased tea leaf (Camellia sinensis L. O. Kuntze). The result implied different cultural activities of spore germination and mycelium growth on different culture media, carbon and nitrogen sources. The pathogens were categorized according to temperature, pH level and salt tolerance capacity. The result indicated fast growth of all the pathogens on Potato Malt Agar medium followed by Potato Dextrose Agar but change in colony morphology. Among the carbon sources tested dextrose combined medium has produced highest mycelium by Pestalotiopsis sp and Colletotrichum gloeosporioides and in case of nitrogen sources tested Ammonium nitrate has yielded highest mycelia followed by malt extract, NaNO?, KNO?, Ammonium sulphate and peptone. The experimental results of physical parameter implied that the pathogen showed batter growth in 25? C to 35?C temperature which can be categorized as mesophiles and among the observed pH level tolerance test maximum growth was observed at pH 5.5. Alternaria alternata and F. solani showed highest tolerance (2.5%) of NaCl salt.