COMPARISON OF CYTOTOXICITY OF BIODENTINE RETROGRADE FILLING  MATERIAL AGAINST MINERAL TRIOXIDE AGGREGATE AND GLASS IONOMER CEMENT� AN INVITRO STUDY.

Dr. Nevil Mathews; PROF (Dr )Mithra N Hegde; PROF(Dr) Priyadarshini Hegde; PROF( Dr.) Veena Shetty

Volume : V, Issue : XI, November - 2015

COMPARISON OF CYTOTOXICITY OF BIODENTINE RETROGRADE FILLING MATERIAL AGAINST MINERAL TRIOXIDE AGGREGATE AND GLASS IONOMER CEMENT� AN INVITRO STUDY.

Dr. Nevil Mathews, Prof Dr Mithra N Hegde, Prof Dr Priyadarshini Hegde, Prof Dr. Veena Shetty

Abstract :

 AIM OF THE STUDY : To compare the cytotoxicity of potential retrograde filling materials against on Human Gingival Fioblasts cell line by means of the Sulforhodamine B assay . MATERIALS AND METHODS : 9 samples each of Biodentine , Mineral trioxide aggregate Angelus and Glass ionomer cement Type IX were prepared for the study and set at 37 0C in 100% relative humidity for one day . The set materials were immersed in Dulbecco Modified Eagle culture medium for 24 hrs. Fioblasts cultured in Dulbecco Modified Eagle Medium were used as a control group. The test materials extracts were then separated and then tested in culture wells in close proximity to growing cell culture and incubated for 24 hrs & 48 hrs. . Cytotoxicity/ Survival fraction was estimated by Sulforhodamine B assay. One way ANOVA test , Bonferroni test and Paired t test were used to evaluate the statistical significance of the results. RESULTS : Cells exposed to extracts from MTA Angelus and Biodentine showed the highest survival fraction percentage after 24 hrs & 48 hrs at all elute concentrations , whereas cells exposed to Glass–ionomer cement type IX extracts displayed the lowest survival fraction percentage. CONCLUSION: On serial dilution of cement extracts , Survival fraction percentage continued to increase on further dilution and showed better results for cell viability and compatibility of all the root filling materials tested. The degree of cytotoxicity in descending order was Glass–ionomer cement type IX, MTA Angelus & Biodentine in the cell line tested for both 24hr and 48 hrs exposure period of the study