Comparison of Photo–Optical and Mechanical  Methods for Prothrombin Time Test

Dr.Deepak Nayak M.; Dr.Chethan Manohar; Mrs. Asha Patil; Saroj

Volume : III, Issue : IX, September - 2013

Comparison of Photo–Optical and Mechanical Methods for Prothrombin Time Test

Dr. Deepak Nayak M. , Dr. Chethan Manohar, Mrs. Asha Patil, Saroj

Abstract :

Background: The PT measures the activity of the so–called extrinsic and common pathways of coagulation. Automated coagulation analyzers have replaced the manual methods to meet the increasing test load in many laboratories. Two distinct automated or semi–automated methods exist based on optical and mechanical clot detection. Aim: To compare the reliability of prothrombin time test using mechanical and photo–optical methods in a semi–automated coagulation analyzer. Materials and methods: Prothrombin time data was collected retrospectively conducted on 100 samples run on mechanical & optical modes on Amax Destiny Plus™ analyzer (Trinity Biotech) in the month of November 2012. The standard deviation (SD), Coefficient of Variance (C.V.) & R² value were calculated. Results: The instrument results showed good precision and coefficient of variation. Statistical analysis demonstrated an excellent correlation between the photo–optical and mechanical methods for PT (R² 0.995). Conclusion: The results for the prothrombin time test obtained by the photo–optical detection method is reliable and is statistically equivalent as those obtained by the mechanical detection method